National Institute Of Genetic Engineering and Biotechnology

P.I:Mohammad-Ali Malboobi
Here we provide last mutation observed in Nucleocapsid gene of COVID-19 prevalent in Iran.
We kindly wait your comment or coopration in tracking crona virus project.
Amino acid changes

How to read this table:
- Rows are reference amino acids and columns are changed amino acids. E.g. Row 'A' column 'E' indicates how many 'A' amino acids have been replaced by 'E' amino acids.
- Red background colors indicate that more changes happened (heat-map).
- Diagonals are indicated using grey background color
- WARNING: This table may include different translation codon tables (e.g. mamalian DNA and mitochondrial DNA).

  * - ? A C D E F G H I K L M N P Q R S T V Y
*     2                                      
-     219                                      
?                                            
A     12 4                             1 3 4  
C     9   1     1                   1        
D     3     1     1 1                       1
E     5     2 1   1     2                    
F     15   2     5         6           2      
G     3   1                           1   1  
H                                            
I     9         1     2   1             1 2  
K     6                 2           1        
L     40         8   1 5   18 1   5 1   1   4  
M     3                                      
N     15     2       1         4       2 1   2
P     5             1     2     4   1 2      
Q                                            
R     9             1 1 1 1         2        
S     22 2 3     4 1   1   1   1 3     8 1   2
T     12 3             2     1         1 6    
V     37 4       6 1   6   7 1             16  
Y     12   1         1                       4

Nucleotide and amino acid mutations

Here we provide list of mutation observed in Nucleocapsid at gene and protein of COVID-19.
-The position column is nucleotide position in reference genome as NC_045512.2
-The Reference column indicate reference nucleotide in NC_045512.2
-The NS column indicate number of samples that this position have been observed
-The NSR column indicate number of samples with reference nucleotide. Therefore diffrenciate related to Ns indicate number of sample with mutated nucleotide
Contoury Position Reference Alt. NSR NS AAchange
0 England 26245 A 190543 190543
1 England 26246 T 190543 190543
2 England 26247 G 190543 190542
3 England 26248 T C 190543 190537 Tyr2His
4 England 26249 A 190543 190543
5 England 26250 C T 190543 190538 Tyr2Tyr
6 England 26251 T C 190543 190539 Ser3Pro
7 England 26252 C T 190543 190541 Ser3Leu
8 England 26253 A 190543 190543
9 England 26254 T C 190544 190538 Phe4Leu
10 England 26255 T 190544 190543
11 England 26256 C T 190545 190363 Phe4Phe
12 England 26257 G A,T 190545 190353 Val5Ile-p.Val5Phe
13 England 26258 T 190545 190544
14 England 26259 T 190545 190544
15 England 26260 T C,G 190545 190540 Ser6Pro-p.Ser6Ala
16 England 26261 C 190545 190541
17 England 26262 G A,T 190545 190506 Ser6Ser-p.Ser6Ser
18 England 26263 G A 190545 190541 Glu7Lys
19 England 26264 A G 190546 190545 Glu7Gly
20 England 26265 A 190546 190546
21 England 26266 G A 190546 190543 Glu8Lys
22 England 26267 A 190546 190546
23 England 26268 G T,A,C 190546 190521 Glu8Asp-p.Glu8Glu
24 England 26269 A T,G 190546 190542 Thr9Ser-p.Thr9Ala
25 England 26270 C T 190546 190156 Thr9Ile
26 England 26271 A T 190546 190544 Thr9Thr
27 England 26272 G T,A 190546 190537 Gly10Cys-p.Gly10Ser
28 England 26273 G T 190546 190542 Gly10Val
29 England 26274 T 190546 190546
30 England 26275 A G 190546 190542 Thr11Ala
31 England 26276 C T 190546 190544 Thr11Met
32 England 26277 G T 190546 190540 Thr11Thr
33 England 26278 T 190546 190545
34 England 26279 T 190546 190545
35 England 26280 A T 190546 190544 Leu12Phe
36 England 26281 A C,G 190546 190537 Ile13Leu-p.Ile13Val
37 England 26282 T 190546 190544
38 England 26283 A T 190546 190541 Ile13Ile
39 England 26284 G 190546 190540
40 England 26285 T 190543 190537
41 England 26286 T C 190543 190538 Val14Val
42 England 26287 A G 190543 190542 Asn15Asp
43 England 26288 A 190543 190543
44 England 26289 T C 190543 190515 Asn15Asn
45 England 26290 A 190540 190540
46 England 26291 G A 190281 190265 Ser16Asn
47 England 26292 C T 190273 190267 Ser16Ser
48 England 26293 G A,T 190272 190264 Val17Ile-p.Val17Leu
49 England 26294 T 190271 190262
50 England 26295 A T,G 190270 190254 Val17Val-p.Val17Val
51 England 26296 C T 190268 190234 Leu18Phe
52 England 26297 T 190268 190255
53 England 26298 T C 190268 190251 Leu18Leu
54 England 26299 C T,A 190268 190244 Leu19Phe-p.Leu19Ile
55 England 26300 T C 190266 190244 Leu19Pro
56 England 26301 T C,G 190266 190246 Leu19Leu-p.Leu19Leu
57 England 26302 T C 190267 190147 Phe20Leu
58 England 26303 T C 190267 190252 Phe20Ser
59 England 26304 T C 190267 190245 Phe20Phe
60 England 26305 C T,G,A 190266 190128 Leu21Val-p.Leu21Ile
61 England 26306 T C 190264 190259 Leu21Pro
62 England 26307 T A 190253 190245 Leu21Leu
63 England 26308 G A 190253 190237 Ala22Thr
64 England 26309 C T 190245 190226 Ala22Val
65 England 26310 T 190242 190234
66 England 26311 T 190242 190235
67 England 26312 T G 190242 190234 Phe23Cys
68 England 26313 C T,A 190242 189218 Phe23Leu-p.Phe23Phe
69 England 26314 G A,T,C 190242 190179 Val24Leu-p.Val24Leu
70 England 26315 T C 190241 190228 Val24Ala
71 England 26316 G T 190230 190214 Val24Val
72 England 26317 G 190229 190217
73 England 26318 T C 190229 190199 Val25Ala
74 England 26319 A G,T 190228 189446 Val25Val-p.Val25Val
75 England 26320 T C 190228 190213 Phe26Leu
76 England 26321 T G 190228 190216 Phe26Cys
77 England 26322 C T,A 190227 190209 Phe26Leu-p.Phe26Phe
78 England 26323 T C 190227 190186 Leu27Leu
79 England 26324 T C 190227 190212 Leu27Ser
80 England 26325 G A,T 190227 190206 Leu27Phe-p.Leu27Leu
81 England 26326 C T,A 190227 190209 Leu28Ile-p.Leu28Leu
82 England 26327 T 190227 190217
83 England 26328 A 190226 190217
84 England 26329 G T 190226 190214 Val29Phe
85 England 26330 T 190226 190203
86 England 26331 T C 190226 190205 Val29Val
87 England 26332 A G 190226 190206 Thr30Ala
88 England 26333 C T 190226 190139 Thr30Ile
89 England 26334 A G,C 190226 190212 Thr30Thr-p.Thr30Thr
90 England 26335 C T,A 190227 190206 Leu31Ile-p.Leu31Leu
91 England 26336 T C 190226 190214 Leu31Pro
92 England 26337 A G 190226 190215 Leu31Leu
93 England 26338 G A 190226 190212 Ala32Thr
94 England 26339 C T 190226 190213 Ala32Val
95 England 26340 C T 190227 190148 Ala32Ala
96 England 26341 A T 190233 190225 Ile33Phe
97 England 26342 T C 190233 190200 Ile33Thr
98 England 26343 C T 190233 190223 Ile33Ile
99 England 26344 C 190233 190224
100 England 26345 T C 190233 190223 Leu34Pro
101 England 26346 T 190233 190224
102 England 26347 A 190232 190223
103 England 26348 C 190232 190222
104 England 26349 T A,C 190232 190218 Thr35Thr-p.Thr35Thr
105 England 26350 G A 190232 190220 Ala36Thr
106 England 26351 C T 190231 190216 Ala36Val
107 England 26352 G T 190230 190217 Ala36Ala
108 England 26353 C T 190230 190195 Leu37Phe
109 England 26354 T A 190230 190219 Leu37His
110 England 26355 T 190231 190223
111 England 26356 C 190231 190222
112 England 26357 G 190231 190225
113 England 26358 A G 190231 190214 Arg38Arg
114 England 26359 T C,G 190231 190209 Leu39Val-p.Leu39Leu
115 England 26360 T 190231 190216
116 England 26361 G 190230 190218
117 England 26362 T 190230 190221
118 England 26363 G 190230 190218
119 England 26364 T 190230 190219
120 England 26365 G T 190237 190215 Ala41Ser
121 England 26366 C T 190237 190211 Ala41Val
122 England 26367 G T,C 190237 190211 Ala41Ala-p.Ala41Ala
123 England 26368 T 190237 190227
124 England 26369 A G 190238 190225 Tyr42Cys
125 England 26370 C T 190237 190211 Tyr42Tyr
126 England 26371 T 190237 190220
127 England 26372 G T 190247 190218 Cys43Phe
128 England 26373 C 190247 190235
129 England 26374 T C 190248 190235 Cys44Arg
130 England 26375 G 190250 190239
131 England 26376 C T 190251 190236 Cys44Cys
132 England 26377 A T 190251 190240 Asn45Tyr
133 England 26378 A 190251 190244
134 England 26379 T 190249 190240
135 England 26380 A G 190249 190164 Ile46Val
136 England 26381 T 190248 190240
137 England 26382 T 190248 190232
138 England 26383 G T 190248 190234 Val47Phe
139 England 26384 T 190248 190237
140 England 26385 T C 190248 190238 Val47Val
141 England 26386 A 190248 190241
142 England 26387 A G 190248 190238 Asn48Ser
143 England 26388 C T 190248 190231 Asn48Asn
144 England 26389 G T 190248 190185 Val49Leu
145 England 26390 T G,C 190248 190235 Val49Gly-p.Val49Ala
146 England 26391 G T,A 190255 190228 Val49Val-p.Val49Val
147 England 26392 A G 190255 190240 Ser50Gly
148 England 26393 G T 190256 190242 Ser50Ile
149 England 26394 T 190256 190245
150 England 26395 C T 190256 190238 Leu51Phe
151 England 26396 T 190256 190244
152 England 26397 T A 190256 190244 Leu51Leu
153 England 26398 G T,A 190256 190219 Val52Leu-p.Val52Ile
154 England 26399 T 190255 190246
155 England 26400 A G 190252 190238 Val52Val
156 England 26401 A 190252 190240
157 England 26402 A G 190252 190223 Lys53Arg
158 England 26403 A G 190252 190240 Lys53Lys
159 England 26404 C T 190252 190236 Pro54Ser
160 England 26405 C T 190252 190235 Pro54Leu
161 England 26406 T G,C 190251 190233 Pro54Pro-p.Pro54Pro
162 England 26407 T 190251 190241
163 England 26408 C T,A,G 190251 190088 Ser55Tyr-p.Ser55Cys
164 England 26409 T C 190251 190227 Ser55Ser
165 England 26410 T C 190251 190239 Phe56Leu
166 England 26411 T C 190251 190243 Phe56Ser
167 England 26412 T C 190251 190242 Phe56Phe
168 England 26413 T 190251 190243
169 England 26414 A 190253 190245
170 England 26415 C T 190255 190248 Tyr57Tyr
171 England 26416 G T,C,A 190255 190188 Val58Leu-p.Val58Ile
172 England 26417 T 190255 190254
173 England 26418 T C 190254 190231 Val58Val
174 England 26419 T 190240 190237
175 England 26420 A 190241 190238
176 England 26421 C T 190239 190232 Tyr59Tyr
177 England 26422 T 190239 190233
178 England 26423 C T 190239 190234 Ser60Phe
179 England 26424 T C,G 190239 185058 Ser60Ser-p.Ser60Ser
180 England 26425 C 190238 190231
181 England 26426 G T,A 190238 190192 Arg61Leu-p.Arg61His
182 England 26427 T 190238 190234
183 England 26428 G T 190238 190070 Val62Phe
184 England 26429 T 190238 190236
185 England 26430 T C 190238 190233 Val62Val
186 England 26431 A 190238 190230
187 England 26432 A 190238 190231
188 England 26433 A G 190238 190228 Lys63Lys
189 England 26434 A G 190236 190233 Asn64Asp
190 England 26435 A C,G 190236 190222 Asn64Thr-p.Asn64Ser
191 England 26436 T C 190236 190234 Asn64Asn
192 England 26437 C T 190235 190226 Leu65Leu
193 England 26438 T 190235 190233
194 England 26439 G T,A 190235 190177 Leu65Leu-p.Leu65Leu
195 England 26440 A T,C 190235 190230 Asn66Tyr-p.Asn66His
196 England 26441 A 190235 190232
197 England 26442 T C 190234 190218 Asn66Asn
198 England 26443 T C 190234 190230 Ser67Pro
199 England 26444 C T,G 190234 190218 Ser67Phe-p.Ser67Cys
200 England 26445 T C 190233 190229 Ser67Ser
201 England 26446 T G,A 190233 190207 Ser68Ala-p.Ser68Thr
202 England 26447 C T,A,G 190233 189960 Ser68Tyr-p.Ser68Cys
203 England 26448 T C 190224 190221 Ser68Ser
204 England 26449 A 190217 190214
205 England 26450 G T,A 190217 190098 Arg69Ile-p.Arg69Lys
206 England 26451 A G 190217 190212 Arg69Arg
207 England 26452 G T,A 190217 190194 Val70Phe-p.Val70Ile
208 England 26453 T C 190215 190211 Val70Ala
209 England 26454 T C 190215 190205 Val70Val
210 England 26455 C T 190214 190192 Pro71Ser
211 England 26456 C T,A,G 190214 189963 Pro71His-p.Pro71Arg
212 England 26457 T C,G 190214 190208 Pro71Pro-p.Pro71Pro
213 England 26458 G C,T 190214 190180 Asp72His-p.Asp72Tyr
214 England 26459 A G 190214 190194 Asp72Gly
215 England 26460 T C 190214 190209 Asp72Asp
216 England 26461 C T,A,G 190214 189971 Leu73Ile-p.Leu73Val
217 England 26462 T C 190214 190209 Leu73Pro
218 England 26463 T G 190214 190208 Leu73Leu
219 England 26464 C T,G,A 190213 189729 Leu74Met-p.Leu74Leu
220 England 26465 T A 190212 190201 Leu74Gln
221 England 26466 G T,A 190208 190175 Leu74Leu-p.Leu74Leu
222 England 26467 G C,A 190208 190172 Val75Leu-p.Val75Ile
223 England 26468 T 190206 190201
224 England 26469 C T,A 190206 190046 Val75Val-p.Val75Val
225 England 26470 T 190205 190205
226 England 26471 A 190204 190204
227 England 26472 A 190202 190202